Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing

Abstract Surface plasmon resonance-based biosensors have been extensively applied to the characterization of binding kinetics between purified (bio)molecules, thanks robust data analysis techniques. However, for solutions containing multiple interactants is still at its infancy. We here present two algorithms (1) reliable and accurate determination kinetic parameters N different ratios in mixtures (2) estimation each interactant a given mixture, assuming that their are known. Both assume compete bind an immobilized ligand 1:1 fashion necessitate prior knowledge total concentration all combined. The effectiveness these was experimentally validated with model system corresponding four small molecular weight drugs protein. This approach enables in-depth using SPR, which may be considerable interest many drug discovery or development applications, notably protein glycovariant analysis.